ABSTRACT
Gastric cancer incidence and morality are high,and its comprehensive treatment of gastric cancer has a major significance in clinical.Riboflavin is a water-soluble vitamin,absorbed by riboflavin transporter protein,which can perform anti-tumor function by inhibiting tumor cell prolifiration and inducing promoting apoptosis.Riboflavin plays importan roles in gastric cancer development,treatment,cancer invasion,metastasis and reducing the adverse effects of chemotherapy.Studying on mechanism and biological characterristic of riboflavin may help to develop more specific individualized treatment plans for the patients with gastric cancer in the future.
ABSTRACT
Objective To study the effect of Abnormal Savda Munziq (ASMq) on MDR and expression of mRNA of Bel/Fu cell line. Methods Several genes expression including MDR1, MRP, TOPOII? and GST-? were analyzed by RT-PCR. Results ASMq has been found to down-regulate MDR1 gene and simultaneously up-regulate TopoⅡ?mRNA. Conclusion ASMq can reverse the drug resistance against 5-Fu in Bel/Fu cells. Inhibition of MDR1 mRNA and up-regulate TopoⅡ? mRNA may contribute to the mechanisms.
ABSTRACT
Objective To investigate the reverse effect of the extracted solution of uygur medicine ASMq on the liver cancer drug-resistance cell line Bel/Fu in vitro.Methods MTT assay was used to determine the IC50 of 5-Fu on Bel/Fu cell line and the reverse multiples of the drug-resistance before and after ASMq treatment.The concentration was used to determine the fold reversal of ASMq.By clone forming text to determine the reversal effect on drug resistance of Bel/Fu cell line.Results The IC50 value of ASMq crude extract was 2 000.68 ?g/mL.ASMq at 390 ?g/mL concentration was no toxic but had a coordinate effect on Bel/Fu cell line with 5-Fu.The fold reversal of ASMq combining with 5-Fu was 7.8,CDI was 0.89.The clone forming size became smaller comply with the concentration of ASMq.Conclusion ASMq has reversal effect on drug resistance of Bel/Fu cell line.